GSK Outreach: Seminar at University of Siena 2017
VIP members Emiliano Chiarot, Charlotte O’Donnell and Mike Stanton recently presented a seminar at the University of Siena. The title of the seminar was “Using in vivo imaging to investigate animal infection models with human bacterial pathogens”.
Emiliano Chiarot opened the seminar by introducing the Vaccine imaging partnership (VIP). He described what each institution brings to the VIP collaboration and how they work together to share knowledge and skills. This was followed by an introduction of the VIP researchers present.
Emiliano then went on to give a brief overview of the tools currently available to perform in vivo imaging and to discuss how imaging is important to implement the 3Rs. He then delved further into optical imaging and described the instruments he uses such as the IVIS to visualize bacteria and inflammation in vivo. Here he showed some examples of imaging both S. aureus in a model of osteomyelitis.
Charlotte followed Emiliano and gave some background on the types of in vivo animal models that are currently used at GSK to model S. aureus infection. In particular she focused on the use of the air-pouch model to simulate skin infection. Charlotte then presented some new data using a bioluminescent probe called Luminol to quantify the early immune response following S. aureus infection.
Mike then went on to discuss research conducted on Listeria by the VIP team that was recently accepted for publication. After a brief introduction to Listeria, Mike presented the click beetle red luciferase reporter system, which can be used to image Listeria infection in vivo. Mike described the benefits of this system compared to the established Lux system.
Finally there were questions from the audience for the VIP members.
GSK Outreach: Siena 2018
Dr Giuliano Bensi, Dr Angela Spagnuolo and Dr Lynn Naughton, all members of the VIP team, attended the two Research & Development days at GSK Siena.